Microsatellite Instability MSI PCR Test
Understanding Microsatellite Instability Testing
Microsatellite Instability (MSI) PCR testing represents a groundbreaking advancement in cancer diagnostics that evaluates the stability of repetitive DNA sequences within tumor cells. This sophisticated molecular analysis detects defects in the DNA mismatch repair system, which normally corrects errors that occur during DNA replication. When this repair mechanism fails, microsatellites—short, repetitive DNA sequences—become unstable and change in length, creating a molecular fingerprint that has profound implications for cancer treatment and prognosis.
What Does the MSI PCR Test Measure?
The MSI PCR test specifically analyzes five critical microsatellite markers through polymerase chain reaction (PCR) and fragment analysis:
- BAT-25 and BAT-26: Mononucleotide repeats that are highly sensitive for detecting MSI
- D2S123, D17S250, and D5S346: Dinucleotide repeats that provide comprehensive instability assessment
This test compares the length patterns of these markers between normal tissue and tumor tissue samples. When two or more markers show instability (changes in length), the result is classified as MSI-High (MSI-H), indicating significant mismatch repair deficiency. This finding has direct clinical implications for treatment selection and patient outcomes.
Who Should Consider MSI Testing?
MSI PCR testing is recommended for individuals in several clinical scenarios:
- Colorectal Cancer Patients: All newly diagnosed colorectal cancer patients should undergo MSI testing to guide treatment decisions
- Endometrial Cancer Patients: Particularly those with specific histological features or family history
- Patients with Suspected Lynch Syndrome: Individuals with personal or family history of Lynch syndrome-associated cancers
- Candidates for Immunotherapy: Patients considering immune checkpoint inhibitor treatments
- Young-Onset Cancer Patients: Individuals diagnosed with cancer before age 50
- Multiple Primary Cancers: Patients with synchronous or metachronous cancers
Clinical Symptoms and Indications
Consider MSI testing if you or your family members experience:
- Early-onset colorectal cancer (before age 50)
- Multiple Lynch syndrome-associated cancers (colorectal, endometrial, gastric, ovarian, etc.)
- Colorectal cancer with specific histological features (mucinous, signet ring cell, etc.)
- Strong family history of colorectal or endometrial cancers
- Poor response to conventional chemotherapy treatments
Benefits of MSI PCR Testing
Undergoing MSI testing provides numerous clinical advantages:
- Immunotherapy Eligibility: MSI-High tumors often respond exceptionally well to immune checkpoint inhibitors
- Treatment Personalization: Guides selection of the most effective therapeutic approaches
- Prognostic Information: MSI status provides valuable insights into disease progression and outcomes
- Hereditary Cancer Risk Assessment: Identifies patients who may benefit from genetic counseling
- Clinical Trial Access: Opens opportunities for targeted therapy trials
- Family Risk Management: Helps identify at-risk family members for preventive screening
Understanding Your Test Results
Your MSI PCR test results will fall into one of three categories:
MSI-High (MSI-H)
This result indicates significant microsatellite instability in two or more markers. MSI-H tumors typically respond well to immunotherapy and may have better prognosis in certain cancer types. Your oncologist will discuss immunotherapy options and potential hereditary cancer syndrome evaluation.
MSI-Low (MSI-L)
Instability in only one marker suggests limited mismatch repair deficiency. Treatment decisions will focus on conventional therapies, though continued monitoring and additional testing may be recommended.
Microsatellite Stable (MSS)
No significant instability detected across all markers. This result indicates intact DNA mismatch repair function, and treatment will typically follow standard protocols for your specific cancer type.
Test Details and Pricing
| Test Component | Details |
|---|---|
| Test Name | Microsatellite Instability MSI PCR Test |
| Regular Price | $500 USD |
| Discount Price | $400 USD |
| Turnaround Time | 10 days |
| Sample Type | FFPE Tissue Block |
| Test Components | BAT-25, BAT-26, D2S123, D17S250, D5S346 |
| Methodology | PCR with Fragment Analysis |
Nationwide Testing Availability
We provide comprehensive MSI PCR testing services across the United States, with convenient locations in all major metropolitan areas including New York, Los Angeles, Chicago, Houston, Phoenix, Philadelphia, San Antonio, San Diego, Dallas, San Jose, and many more. Our network of certified laboratories ensures consistent, high-quality results regardless of your location.
Take Control of Your Cancer Treatment Journey
MSI testing represents a critical step in modern cancer care, providing essential information that can significantly impact your treatment outcomes. With our discounted price of $400 and rapid 10-day turnaround, there’s no reason to delay this important diagnostic evaluation.
Ready to schedule your Microsatellite Instability MSI PCR Test? Contact our dedicated oncology testing specialists today at +1(267) 388-9828 or book your appointment online. Our team is available to answer your questions, assist with sample coordination, and ensure you receive the comprehensive cancer care you deserve.
