Immunophenotyping by Flow Cytometry CD34 Stem Cell Enumeration Test
Comprehensive Introduction to CD34 Stem Cell Testing
The Immunophenotyping by Flow Cytometry CD34 Stem Cell Enumeration Test represents a cornerstone in modern hematologic diagnostics and transplantation medicine. This advanced laboratory procedure provides precise quantification of CD34-positive hematopoietic stem cells, which serve as the fundamental building blocks for blood cell production and immune system function. The test’s importance extends across multiple medical specialties, particularly in oncology and transplantation pathology, where accurate stem cell assessment directly impacts treatment decisions and patient outcomes.
CD34 is a specific cell surface marker expressed on primitive hematopoietic stem and progenitor cells. These cells possess the remarkable ability to differentiate into all blood cell lineages, including red blood cells, white blood cells, and platelets. The enumeration of CD34+ cells has become an essential parameter in clinical practice, guiding critical decisions in stem cell transplantation, chemotherapy monitoring, and hematologic disease management.
What the Test Measures and Detects
This sophisticated diagnostic test specifically identifies and quantifies CD34-positive stem cells using state-of-the-art flow cytometry technology. The test measures:
- Absolute CD34+ Cell Count: Precise enumeration of CD34-expressing stem cells per microliter of sample
- Stem Cell Viability: Assessment of cell health and functional capacity
- Purity of Stem Cell Population: Evaluation of the proportion of CD34+ cells within the total cell population
- Stem Cell Mobilization Efficiency: Monitoring response to growth factor therapy
The test utilizes multiparameter flow cytometry with fluorescent-labeled antibodies that specifically bind to CD34 antigens, allowing for accurate identification and counting of these critical stem cells. This technology provides superior sensitivity and specificity compared to traditional microscopic methods.
Who Should Consider This Test
Clinical Indications and Patient Populations
This specialized testing is recommended for patients in several clinical scenarios:
- Stem Cell Transplant Candidates: Patients undergoing autologous or allogeneic stem cell transplantation
- Cancer Patients: Individuals receiving chemotherapy for hematologic malignancies
- Peripheral Blood Stem Cell Collection: Patients undergoing apheresis for stem cell harvesting
- Bone Marrow Failure Syndromes: Conditions like aplastic anemia or myelodysplastic syndromes
- Treatment Monitoring: Patients receiving granulocyte colony-stimulating factor (G-CSF) therapy
- Cord Blood Banking: Assessment of stem cell content in umbilical cord blood samples
Symptoms and Clinical Presentations
Patients may present with various symptoms that warrant CD34 stem cell enumeration:
- Unexplained cytopenias (low blood counts)
- Poor bone marrow function recovery post-chemotherapy
- Delayed engraftment following stem cell transplantation
- Monitoring stem cell mobilization protocols
- Evaluation of graft quality for transplantation
Benefits of CD34 Stem Cell Enumeration Testing
This advanced diagnostic procedure offers numerous clinical advantages:
- Precision Treatment Planning: Enables accurate dosing of stem cells for transplantation
- Improved Transplant Outcomes: Helps predict engraftment success and timing
- Treatment Optimization: Guides appropriate timing for stem cell collection
- Risk Assessment: Identifies patients at risk for poor mobilization
- Quality Control: Ensures adequate stem cell doses for successful transplantation
- Cost-Effective Care: Prevents unnecessary procedures and optimizes resource utilization
Understanding Your Test Results
Interpretation Guidelines
Test results provide critical information for clinical decision-making:
- Optimal Range: CD34+ cell counts typically range from 20-500 cells/μL in peripheral blood during mobilization
- Transplantation Threshold: Minimum required CD34+ cell dose varies by transplant type (usually 2-5 million cells/kg)
- Mobilization Success: Adequate mobilization generally requires >10-20 CD34+ cells/μL in peripheral blood
- Clinical Correlation: Results must be interpreted in context with clinical presentation and treatment history
Your healthcare provider will explain the specific implications of your results and how they relate to your treatment plan. Normal ranges may vary based on the laboratory methodology and patient factors.
Test Pricing and Availability
| Price Type | Amount (USD) |
|---|---|
| Discount Price | $88 |
| Regular Price | $112 |
Sample Requirements and Turnaround Time
- Sample Types: Whole blood (EDTA and Sodium Heparin tubes), Bone marrow, Cord blood, or Apheresis samples
- Volume Requirements: 3 mL whole blood minimum, 2 mL bone marrow minimum, or 1 mL cord blood minimum
- Shipping Instructions: Ship immediately at appropriate temperatures (18-22°C or 2-8°C depending on sample type)
- Turnaround Time: Sample daily by 9 AM; Report same day
- Pre-test Instructions: Provide brief clinical history for optimal interpretation
Nationwide Testing Availability
GGC DNA maintains comprehensive testing facilities across the United States, with branches conveniently located in all major metropolitan areas including New York, Los Angeles, Chicago, Houston, Phoenix, Philadelphia, San Antonio, San Diego, Dallas, San Jose, and many other cities. Our network ensures accessible, high-quality CD34 stem cell enumeration testing for patients nationwide.
Book Your CD34 Stem Cell Enumeration Test Today
Take the next step in your hematologic health management with our advanced CD34 stem cell enumeration testing. Our specialized flow cytometry laboratory provides accurate, reliable results that inform critical treatment decisions. Contact us today to schedule your test and receive the comprehensive stem cell assessment you need for optimal care.
Call or WhatsApp: +1(267) 388-9828
Our experienced team is ready to assist you with test scheduling, sample collection guidance, and result interpretation. Trust GGC DNA for premier diagnostic testing that makes a difference in patient care.
