Immunohistochemistry PAX5 Test: Advanced B-Cell Lineage Detection
Understanding the PAX5 Immunohistochemistry Test
The Immunohistochemistry PAX5 Test represents a cutting-edge diagnostic approach in modern oncology and pathology. This specialized laboratory procedure detects the presence of PAX5 protein, a transcription factor that plays a critical role in B-cell development and differentiation. As a nuclear marker expressed throughout B-cell maturation, PAX5 serves as an essential tool for identifying B-cell lineage in various hematological malignancies and solid tumors.
The importance of this test cannot be overstated in contemporary cancer diagnosis. PAX5 expression is maintained in most B-cell lymphomas but is typically absent in T-cell lymphomas and other non-hematopoietic tumors. This distinctive pattern makes it invaluable for differential diagnosis and proper classification of lymphoid malignancies, ultimately guiding targeted treatment strategies that can significantly impact patient outcomes.
What the PAX5 Test Measures and Detects
The Immunohistochemistry PAX5 Test specifically identifies the presence and distribution of PAX5 protein within tissue samples. This sophisticated detection method provides crucial information about:
- B-cell lineage confirmation in suspected lymphoma cases
- Differentiation between B-cell and T-cell lymphomas
- Identification of precursor B-lymphoblastic leukemia/lymphoma
- Detection of classical Hodgkin lymphoma where PAX5 is typically weakly expressed
- Distinction of B-cell neoplasms from other tumor types
Technical Methodology
This test utilizes advanced immunohistochemistry techniques where tissue sections are incubated with specific antibodies that bind to PAX5 protein. The resulting staining pattern is then examined under microscopy by experienced pathologists to determine the presence, intensity, and distribution of PAX5 expression within the tissue architecture.
Who Should Consider the PAX5 Immunohistochemistry Test?
This specialized diagnostic test is recommended for patients presenting with specific clinical scenarios and symptoms that suggest potential lymphoid malignancies:
Clinical Indications
- Patients with unexplained lymphadenopathy (swollen lymph nodes)
- Individuals with suspected lymphoma based on imaging studies
- Cases where initial biopsy results are inconclusive for lymphoma classification
- Patients with B-symptoms (fever, night sweats, weight loss) of unknown origin
- Suspected hematological malignancies with atypical presentation
- Differentiation between reactive lymphoid hyperplasia and lymphoma
Referring Specialties
This test is typically ordered by:
- Oncologists managing patients with suspected blood cancers
- Pathologists requiring additional markers for definitive diagnosis
- Hematologists evaluating abnormal blood cell populations
- Surgeons performing biopsies of suspicious masses
Benefits of Taking the PAX5 Immunohistochemistry Test
Choosing the PAX5 Immunohistochemistry Test provides numerous advantages for both patients and healthcare providers:
Diagnostic Precision
- Accurate classification of lymphoid malignancies
- Definitive B-cell lineage determination for treatment planning
- Reduced diagnostic uncertainty in complex cases
- Enhanced differential diagnosis between similar appearing tumors
Clinical Impact
- Informed treatment decisions based on precise tumor classification
- Potential for targeted therapies in specific lymphoma subtypes
- Prognostic information that guides management strategies
- Monitoring disease progression and treatment response
Patient Advantages
- Comprehensive diagnostic evaluation in a single test
- Rapid turnaround time with results typically available in 5 days
- Nationwide accessibility through our network of testing centers
- Cost-effective pricing with significant discounts available
Understanding Your PAX5 Test Results
Interpreting PAX5 Immunohistochemistry results requires specialized expertise, but understanding the general principles can help patients comprehend their diagnosis:
Positive PAX5 Expression
A positive result indicates the presence of PAX5 protein in the nuclei of tumor cells, strongly suggesting B-cell lineage. This finding typically supports diagnoses such as:
- B-cell lymphomas (various subtypes)
- B-lymphoblastic leukemia/lymphoma
- Some cases of classical Hodgkin lymphoma (usually weak expression)
Negative PAX5 Expression
Absence of PAX5 staining may indicate:
- T-cell lymphomas
- Non-hematopoietic tumors
- Plasma cell neoplasms (which lose PAX5 expression)
- Certain mature B-cell lymphomas with aberrant marker loss
Interpretation Context
It’s crucial to understand that PAX5 results are interpreted in conjunction with other clinical, morphological, and immunohistochemical findings. Your pathologist and oncologist will integrate this information with other test results to provide a comprehensive diagnosis and treatment recommendation.
Test Pricing and Availability
| Test Name | Discount Price | Regular Price |
|---|---|---|
| Immunohistochemistry PAX5 Test | $88 USD | $126 USD |
Turnaround Time
- Sample Submission: Daily by 6 PM
- Standard Tissue Block: 5 days
- Tissue Biopsy: 5 days
- Large Complex Tissue: 7 days
Sample Requirements
For accurate testing, please provide:
- Tumor tissue in 10% formal-saline OR formalin-fixed paraffin-embedded block
- Ship at room temperature with appropriate packaging
- Include copy of Histopathology report
- Document site of biopsy and complete clinical history
Nationwide Testing Network
We proudly serve patients across the United States with testing facilities in all major metropolitan areas including New York, Los Angeles, Chicago, Houston, Phoenix, Philadelphia, San Antonio, San Diego, Dallas, San Jose, Austin, Jacksonville, Fort Worth, Columbus, Charlotte, San Francisco, Indianapolis, Seattle, Denver, Washington DC, Boston, El Paso, Nashville, Detroit, Oklahoma City, Portland, Las Vegas, Memphis, Louisville, Baltimore, and Milwaukee.
Book Your PAX5 Immunohistochemistry Test Today
Take the next step toward accurate diagnosis and effective treatment planning. Our experienced team is ready to assist you with scheduling, sample collection guidance, and result interpretation.
Call or WhatsApp us today at +1(267) 388-9828 to schedule your test or speak with our genetic counseling specialists.
Early and accurate diagnosis through advanced testing like the PAX5 Immunohistochemistry Test can make a significant difference in treatment outcomes. Don’t delay – contact us now to begin your diagnostic journey with confidence and clarity.
