Bacterial Genome Sequencing De Novo Assembly and Annotation Illumina and ONT Hybrid

Comprehensive Introduction to Advanced Bacterial Genome Analysis

Bacterial genome sequencing represents the cutting edge of microbial genetic analysis, providing unprecedented insights into bacterial biology, pathogenicity, and evolutionary mechanisms. Our specialized De Novo Assembly and Annotation service combines two powerful sequencing technologies—Illumina’s high-accuracy short-read sequencing and Oxford Nanopore Technologies’ (ONT) long-read sequencing—to deliver complete, high-quality bacterial genome assemblies without relying on reference genomes.

This hybrid approach revolutionizes how researchers and clinicians understand bacterial genetics by overcoming the limitations of single-technology sequencing. The integration of Illumina’s precision with ONT’s ability to sequence long DNA fragments enables comprehensive genome characterization, making it an indispensable tool for modern microbiology and infectious disease research.

What This Advanced Test Measures and Detects

Our comprehensive bacterial genome sequencing service provides detailed analysis across multiple genetic dimensions:

Complete Genome Assembly

• De novo assembly of complete bacterial genomes without reference bias
• Identification of structural variants and genomic rearrangements
• Detection of plasmid content and mobile genetic elements
• Assembly of repetitive regions and complex genomic structures

Comprehensive Genetic Annotation

• Precise identification of protein-coding genes and open reading frames
• Annotation of non-coding RNA genes and regulatory elements
• Characterization of promoter regions and transcription factor binding sites
• Identification of operons and gene clusters

Functional Analysis

• Prediction of metabolic pathways and biochemical functions
• Identification of virulence factors and pathogenicity islands
• Detection of antibiotic resistance genes and mechanisms
• Analysis of stress response and adaptation genes

Who Should Consider This Advanced Bacterial Analysis

This sophisticated genomic service is essential for multiple professional domains and research applications:

Clinical and Research Applications

• Infectious disease researchers studying bacterial pathogenesis
• Public health laboratories tracking outbreak strains
• Pharmaceutical companies developing new antibiotics
• Academic institutions conducting microbial genomics research
• Biotechnology firms engineering microbial strains

Specific Use Cases

• Outbreak investigation and strain typing
• Antibiotic resistance mechanism studies
• Vaccine development research
• Microbial evolution and adaptation studies
• Comparative genomics across bacterial species

Significant Benefits of Comprehensive Bacterial Genome Sequencing

Choosing our hybrid sequencing approach provides numerous advantages for bacterial genetic analysis:

Unparalleled Data Quality

• Combined accuracy of Illumina sequencing with long-range context from ONT
• Complete genome assemblies without gaps or missing regions
• High-confidence variant calling and structural variant detection
• Comprehensive coverage of complex genomic regions

Research and Clinical Applications

• Enables discovery of novel genes and regulatory elements
• Provides insights into bacterial adaptation and evolution
• Supports development of targeted therapeutic approaches
• Facilitates understanding of host-pathogen interactions

Technical Advantages

• Eliminates reference bias in genome assembly
• Detects epigenetic modifications through ONT sequencing
• Provides phased haplotypes for heterozygous regions
• Enables real-time data analysis during sequencing runs

Understanding Your Comprehensive Results

Our detailed analysis provides comprehensive insights into bacterial genetics with clear, actionable results:

Primary Deliverables

• Complete assembled genome in standard file formats (FASTA, GenBank)
• Comprehensive annotation files with gene predictions and functional assignments
• Quality control metrics and assembly statistics
• Comparative analysis against reference databases when applicable

Interpretation Guidance

• Detailed reports on identified virulence factors and antibiotic resistance genes
• Functional pathway analysis and metabolic reconstruction
• Comparative genomics insights against related bacterial strains
• Technical support for result interpretation and downstream analysis

Application Support

• Guidance on integrating results into research projects
• Support for publication-quality figure generation
• Assistance with database submissions and data sharing
• Consultation on follow-up experiments and validation studies

Test Pricing and Service Details

Turnaround Time: 8 weeks
Sample Type: Extracted DNA

Nationwide Availability and Booking Information

We proudly serve researchers and institutions across the United States with our comprehensive bacterial genome sequencing services. Our state-of-the-art facilities are strategically located in major metropolitan areas including New York, Los Angeles, Chicago, Houston, Phoenix, and Philadelphia, ensuring convenient access to cutting-edge genomic analysis.

Our experienced team of genomic scientists and bioinformaticians is dedicated to providing exceptional service and support throughout your research project. From sample submission to final data delivery, we maintain the highest standards of quality and accuracy.

Ready to Advance Your Bacterial Research?

Take the next step in understanding bacterial genetics with our comprehensive sequencing service. Our team is ready to assist you with sample requirements, project planning, and technical consultation.

Call or WhatsApp us today at +1(267) 388-9828 to schedule your bacterial genome sequencing service or to discuss your specific research needs with our genomics specialists.

Transform your bacterial research with complete genome insights and advanced annotation capabilities. Contact us now to begin your journey into comprehensive microbial genomics.