CD34 Immunophenotyping by Flow Cytometry Test
Comprehensive Introduction to CD34 Immunophenotyping
CD34 Immunophenotyping by Flow Cytometry represents a cornerstone in modern hematological diagnostics, providing clinicians with essential information about hematopoietic stem cell populations. This sophisticated laboratory technique enables precise identification and quantification of CD34-positive cells, which serve as critical markers for bone marrow function and blood cell regeneration capacity. The test’s importance extends across multiple medical specialties, particularly in oncology and hematology, where accurate stem cell assessment directly impacts treatment decisions and patient outcomes.
Flow cytometry technology allows for the simultaneous analysis of multiple cell surface markers, making CD34 immunophenotyping an invaluable tool for characterizing stem cell populations. The CD34 antigen, a transmembrane phosphoglycoprotein, serves as a reliable marker for primitive hematopoietic progenitor cells and endothelial cells. Its detection provides crucial insights into bone marrow health, transplantation success, and various hematological malignancies.
What the CD34 Test Measures and Detects
The CD34 Immunophenotyping test specifically measures:
- Quantification of CD34-positive hematopoietic stem cells in peripheral blood or bone marrow
- Percentage of CD34+ cells within the total nucleated cell population
- Absolute CD34+ cell counts per microliter of sample
- Assessment of stem cell mobilization and engraftment potential
- Detection of abnormal CD34 expression patterns in hematological malignancies
This advanced diagnostic approach utilizes multiple fluorochrome-conjugated antibodies to identify CD34+ cells while excluding other cell types, ensuring highly specific and accurate results. The technology can distinguish between different maturation stages of hematopoietic cells, providing comprehensive information about bone marrow function and regeneration capacity.
Who Should Consider CD34 Immunophenotyping Testing
Clinical Indications and Symptoms
Patients experiencing the following conditions or symptoms may benefit from CD34 immunophenotyping:
- Individuals undergoing evaluation for hematological cancers including leukemia and lymphoma
- Patients preparing for autologous or allogeneic stem cell transplantation
- Those with unexplained cytopenias or bone marrow failure syndromes
- Individuals monitoring stem cell mobilization following chemotherapy or growth factor therapy
- Patients with suspected myelodysplastic syndromes or myeloproliferative disorders
- Those requiring assessment of bone marrow recovery post-chemotherapy
Specific Symptoms Warranting Testing
- Unexplained fatigue, weakness, or pallor suggesting bone marrow dysfunction
- Recurrent infections indicating possible immune system compromise
- Unexplained bruising or bleeding tendencies
- Abnormal complete blood count results requiring further investigation
- Monitoring requirements for stem cell transplant recipients
Significant Benefits of CD34 Immunophenotyping
Undergoing CD34 immunophenotyping provides numerous clinical advantages:
- Precision Diagnosis: Enables accurate identification and quantification of hematopoietic stem cells critical for proper diagnosis
- Treatment Guidance: Provides essential data for stem cell transplantation planning and timing
- Monitoring Capability: Allows tracking of stem cell mobilization and engraftment success
- Prognostic Value: Offers valuable prognostic information for various hematological conditions
- Rapid Results: Same-day turnaround when samples are submitted by 9 AM
- Comprehensive Analysis: Multi-parameter assessment provides detailed hematopoietic profile
Understanding Your CD34 Test Results
Interpretation Guidelines
CD34 immunophenotyping results require careful interpretation by qualified hematologists or oncologists. Typical result patterns include:
Normal CD34+ Cell Ranges
- Peripheral blood: Typically less than 0.1% of nucleated cells
- Bone marrow: Usually 1-3% of nucleated cells
- Absolute counts vary based on clinical context and mobilization status
Clinical Significance of Results
- Elevated CD34+ counts: May indicate active stem cell mobilization, certain leukemias, or bone marrow regeneration
- Decreased CD34+ counts: Can suggest bone marrow failure, extensive chemotherapy damage, or certain hematological disorders
- Abnormal CD34 expression patterns: May indicate hematological malignancies requiring further investigation
It’s crucial to discuss your specific results with your healthcare provider, as interpretation depends on individual clinical circumstances, treatment history, and concurrent laboratory findings.
Test Pricing and Availability
| Test Description | Regular Price | Discount Price |
|---|---|---|
| CD34 Immunophenotyping by Flow Cytometry | $112 USD | $88 USD |
Sample Requirements and Processing
- Sample Types: 3 mL whole blood in EDTA tube AND 3 mL whole blood in Sodium Heparin tube OR 2 mL bone marrow in Sodium Heparin tube
- Turnaround Time: Sample daily by 9 AM; Report same day
- Shipping Instructions: Ship immediately at 18-22°C. DO NOT REFRIGERATE OR FREEZE
- Documentation: Specify time, date, and clinical details on test request form
Nationwide Testing Availability
GGC DNA maintains comprehensive testing facilities across the United States, ensuring accessible CD34 immunophenotyping services in all major metropolitan areas. Our network includes specialized collection centers in:
- New York City and surrounding tri-state area
- Los Angeles and Southern California region
- Chicago and Midwest territories
- Houston, Dallas, and Texas locations
- Miami, Orlando, and Florida centers
- Philadelphia, Boston, Washington DC, and additional East Coast facilities
Each location adheres to strict quality control standards and employs experienced phlebotomists trained in proper sample collection techniques for flow cytometry testing.
Schedule Your CD34 Immunophenotyping Test Today
Take the next step in comprehensive hematological assessment by scheduling your CD34 immunophenotyping test. Our dedicated team ensures prompt sample processing and accurate results to support your healthcare journey. For immediate scheduling or questions about the testing process, contact our patient services department.
Call or WhatsApp: +1(267) 388-9828
Our knowledgeable staff can assist with appointment scheduling, provide detailed preparation instructions, and address any concerns about the testing process. We work closely with healthcare providers to ensure seamless integration of test results into your overall care plan.
Early and accurate diagnosis through CD34 immunophenotyping can significantly impact treatment outcomes for hematological conditions. Don’t delay essential diagnostic testing – book your appointment today and take control of your hematological health with confidence.
