CD33 Immunophenotyping by Flow Cytometry Test
Comprehensive Introduction to CD33 Immunophenotyping
CD33 Immunophenotyping by Flow Cytometry represents a cutting-edge diagnostic approach in modern hematology and oncology. This sophisticated test specifically targets the CD33 antigen, a crucial cell surface marker predominantly expressed on myeloid cells. The clinical significance of CD33 detection extends far beyond simple identification, providing essential insights into disease characterization, treatment selection, and therapeutic monitoring for various hematological malignancies.
Flow cytometry technology enables precise quantification of CD33 expression at the single-cell level, offering unprecedented accuracy in distinguishing normal from malignant cell populations. This advanced methodology has revolutionized the diagnostic landscape for acute myeloid leukemia (AML) and related disorders, allowing clinicians to make evidence-based decisions regarding targeted therapy approaches.
What the CD33 Test Measures and Detects
The CD33 Immunophenotyping test specifically measures the presence and density of CD33 antigens on the surface of blood and bone marrow cells. This transmembrane protein, also known as Siglec-3, serves as a critical marker for myeloid lineage cells and plays a significant role in immune regulation and cell signaling.
Key Detection Capabilities:
- Identification of CD33-positive blast populations in acute myeloid leukemia
- Quantification of CD33 expression levels for treatment planning
- Detection of aberrant CD33 expression patterns in myelodysplastic syndromes
- Monitoring CD33 expression changes during therapy
- Assessment of minimal residual disease in treated patients
Who Should Consider CD33 Immunophenotyping Testing
This specialized test is particularly important for individuals presenting with symptoms or clinical findings suggestive of hematological malignancies. Patients who may benefit from CD33 testing include:
Clinical Indications:
- Patients with unexplained cytopenias (low blood counts)
- Individuals with suspected acute myeloid leukemia based on peripheral blood findings
- Patients with bone marrow abnormalities detected during evaluation
- Individuals undergoing evaluation for myelodysplastic syndromes
- Patients requiring monitoring during targeted CD33-directed therapy
- Those with suspected minimal residual disease following treatment
Symptoms That May Warrant Testing:
- Unexplained fatigue and weakness
- Frequent infections or fever
- Easy bruising or bleeding tendencies
- Paleness or shortness of breath
- Bone pain or tenderness
- Unexplained weight loss
Significant Benefits of CD33 Immunophenotyping
The CD33 Immunophenotyping test offers numerous clinical advantages that significantly impact patient care and treatment outcomes:
Diagnostic Precision:
- Provides definitive identification of myeloid lineage involvement
- Enables accurate classification of leukemia subtypes
- Supports differential diagnosis between similar hematological conditions
- Facilitates appropriate treatment selection based on CD33 expression
Therapeutic Guidance:
- Identifies candidates for CD33-targeted therapies like gemtuzumab ozogamicin
- Monitors treatment response and effectiveness
- Detects early signs of disease progression or relapse
- Guides decisions regarding stem cell transplantation
Prognostic Value:
- Provides valuable prognostic information for treatment planning
- Helps predict response to specific therapeutic approaches
- Supports risk stratification and personalized treatment strategies
- Enables long-term disease monitoring and management
Understanding Your CD33 Test Results
Interpreting CD33 Immunophenotyping results requires specialized expertise, but understanding the general principles can help patients engage more effectively in their care:
Normal Findings:
Normal CD33 expression typically shows appropriate distribution on mature myeloid cells and their precursors, with expected intensity patterns that vary according to cell maturation stage.
Abnormal Patterns:
- Increased CD33 Expression: Often associated with acute myeloid leukemia, particularly in certain subtypes
- Aberrant CD33 Patterns: May indicate myelodysplastic syndromes or other hematological disorders
- CD33 Negative Blasts: Can suggest specific leukemia subtypes with different treatment implications
- Heterogeneous Expression: May indicate clonal evolution or treatment resistance
Clinical Correlation:
Your hematologist or oncologist will interpret your CD33 results in the context of your complete clinical picture, including other laboratory findings, symptoms, and medical history. The results help guide treatment decisions and provide valuable information about disease characteristics and potential therapeutic targets.
Test Pricing and Availability
| Price Type | Amount (USD) |
|---|---|
| Discount Price | $88 |
| Regular Price | $112 |
Turnaround Time and Sample Requirements
Turnaround: Sample Daily by 9 AM; Report Same Day
Sample Type: 3 mL (2 mL min.) whole blood in 1 Lavender Top (EDTA) tube AND 3 mL (2 mL min.) whole blood in 1 Green Top (Sodium Heparin) tube OR 2 mL (1 mL min.) Bone marrow in 1 Green Top (Sodium heparin) tube.
Shipping Instructions: Ship immediately at 18-22°C. DO NOT REFRIGERATE OR FREEZE. Specify time, date and clinical details on test request form.
Nationwide Testing Availability
GGC DNA maintains comprehensive testing facilities across the United States, ensuring convenient access to advanced CD33 immunophenotyping services. Our network includes specialized centers in major metropolitan areas including:
- New York City and surrounding regions
- Los Angeles and Southern California
- Chicago and the Midwest
- Houston and Texas medical centers
- Miami and Florida healthcare facilities
- Boston and New England areas
- San Francisco Bay Area
- Washington D.C. and Baltimore regions
Take Action for Your Health
Don’t delay critical diagnostic testing that could significantly impact your treatment journey. The CD33 Immunophenotyping test provides essential information that can guide life-saving therapeutic decisions and monitor treatment effectiveness.
Ready to schedule your CD33 Immunophenotyping test? Contact our specialized team today to discuss your testing needs and arrange sample collection at a location convenient for you.
Call or WhatsApp: +1(267) 388-9828
Our experienced healthcare professionals are available to answer your questions, explain the testing process, and help you take the next step toward comprehensive hematological evaluation. With same-day results and nationwide availability, GGC DNA makes advanced diagnostic testing accessible when you need it most.
