Immunohistochemistry BRAF V600E Test
Understanding the BRAF V600E Mutation Test
The Immunohistochemistry BRAF V600E Test represents a cutting-edge approach in cancer diagnostics, specifically designed to detect the presence of the BRAF V600E mutation in tumor tissues. This mutation occurs in the BRAF gene, which plays a critical role in regulating cell growth and division. When this gene mutates, it can lead to uncontrolled cell proliferation, contributing to cancer development and progression.
Immunohistochemistry (IHC) provides a sophisticated method for visualizing specific proteins within tissue samples. For the BRAF V600E test, specialized antibodies are used to detect the mutated BRAF protein directly in cancer cells. This approach offers several advantages over traditional genetic testing methods, including the ability to analyze protein expression patterns and provide spatial information about mutation distribution within tumor tissues.
What Does the Test Detect?
The Immunohistochemistry BRAF V600E Test specifically identifies the presence of the V600E mutation in the BRAF gene. This particular mutation involves a single nucleotide change that results in the substitution of valine with glutamic acid at position 600 of the BRAF protein. The test measures:
- Presence of mutated BRAF V600E protein in tumor cells
- Distribution and intensity of mutation expression within tissue samples
- Cellular localization of the mutated protein
- Percentage of tumor cells expressing the BRAF V600E mutation
Who Should Consider This Test?
Clinical Indications and Symptoms
This test is particularly important for patients diagnosed with or suspected of having certain types of cancer where BRAF mutations are commonly found. Consider this test if you have:
- Diagnosed melanoma, especially advanced or metastatic cases
- Colorectal cancer with specific histological features
- Thyroid cancer, particularly papillary thyroid carcinoma
- Non-small cell lung cancer with adenocarcinoma histology
- Hairy cell leukemia or other hematological malignancies
- Failed initial treatment responses requiring alternative therapeutic options
Patient Populations That Benefit Most
The test is especially valuable for patients who may be candidates for targeted therapy. Those with aggressive disease courses, treatment-resistant cancers, or rare tumor types often benefit from BRAF mutation testing to guide personalized treatment approaches.
Benefits of BRAF V600E Testing
Personalized Treatment Planning
Detection of the BRAF V600E mutation opens doors to targeted therapies specifically designed to inhibit the mutated BRAF protein. These targeted treatments often demonstrate superior efficacy compared to conventional chemotherapy, with potentially fewer side effects.
Improved Prognostic Information
Understanding your BRAF mutation status provides valuable prognostic information. Patients with BRAF mutations may have different disease courses and treatment responses, allowing for more accurate predictions about disease progression and outcomes.
Therapeutic Decision Support
The test results directly influence treatment selection. Positive BRAF V600E findings may qualify patients for FDA-approved targeted therapies, while negative results help avoid unnecessary treatments and associated costs.
Comprehensive Cancer Management
By integrating BRAF mutation status with other clinical and pathological findings, healthcare providers can develop more comprehensive treatment strategies tailored to individual patient needs.
Understanding Your Test Results
Interpreting BRAF V600E Findings
Your test results will typically fall into one of several categories:
- Positive for BRAF V600E Mutation: Indicates the presence of the mutation in tumor cells, making you a potential candidate for targeted BRAF inhibitor therapy.
- Negative for BRAF V600E Mutation: Suggests the absence of this specific mutation, though other BRAF mutations may be present that require different testing methods.
- Equivocal Results: May require additional testing or correlation with clinical findings for accurate interpretation.
Clinical Implications
A positive BRAF V600E result typically indicates eligibility for targeted therapies such as vemurafenib, dabrafenib, or encorafenib. These medications specifically target the mutated BRAF protein, potentially leading to improved treatment responses and survival outcomes.
Next Steps After Testing
Your oncologist will discuss the implications of your test results and develop a personalized treatment plan based on the findings. Regular follow-up and monitoring will be essential to assess treatment response and manage any side effects.
Test Pricing and Availability
| Test Name | Discount Price | Regular Price |
|---|---|---|
| Immunohistochemistry BRAF V600E Test | $88 USD | $126 USD |
Sample Requirements and Processing
Sample Collection Guidelines
For accurate BRAF V600E testing, we require:
- Tumor tissue preserved in 10% formal-saline solution
- Formalin-fixed paraffin-embedded tissue blocks
- Shipment at room temperature with proper packaging
- Complete clinical documentation including histopathology report
- Detailed biopsy site information and clinical history
Turnaround Times
- Sample acceptance: Daily by 6 PM
- Tissue blocks: 5 days for results
- Tissue biopsy samples: 5 days for results
- Large complex tissue specimens: 7 days for results
Nationwide Testing Availability
GGC DNA maintains testing facilities across major metropolitan areas throughout the United States, ensuring convenient access to advanced cancer diagnostics. Our network includes locations in New York, Los Angeles, Chicago, Houston, Phoenix, Philadelphia, San Antonio, San Diego, Dallas, and San Jose, among other cities.
Take Control of Your Cancer Treatment Journey
Understanding your BRAF mutation status represents a crucial step toward personalized cancer care. With targeted therapies becoming increasingly available, this test provides essential information that can significantly impact your treatment outcomes and quality of life.
Ready to schedule your Immunohistochemistry BRAF V600E Test? Our dedicated team is available to answer your questions and guide you through the testing process. Contact us today to book your appointment and take the first step toward personalized cancer treatment.
Call or WhatsApp: +1(267) 388-9828
Our experienced oncology specialists and genetic counselors are standing by to provide comprehensive support and ensure you receive the highest quality diagnostic services available.
